ABSTRACT
ATP-diphosphohydrolase (apyrase, EC 3.6.1.5) has both ATPase and ADPase activity that are stimulated by bivalent metais, with Ca2+ being the most effective. The possible physiological function of this enzyme, associated with placental and renal microvilli, is related to the extracellular metabolism of nucleotides. A comparison of the biochemical properties of human placenta and rat kidney apyrase is presented, showing similaiities in Mr, bivalent metal stimulation, nucleotide nonspecificity, insensitivity towards specifjc ATPase inhibitors, and lack of essential sulfhydryl and aliphatic hydroxyl groups. We describe the treatment of membrane preparations from both tissues with different detergents and the isoelectric focusing of the solubilized proteins to partially purify apyrase. An ectoenzyme localization is assigned both in microvillus membranes and in the vasculature on the basis of organ perfusion experiments with nucleotides in the presence of antibodies. Placental and kidney microvillus membranes inhibited ADP-induced platelet aggregation, in agreement with an extracellular role. Initial studies on enzyme regulation suggested the existence of at least two types of modulatory proteins: an activating protein in the cytosol of both tissues, and an inhibitory protein associated with placental microsomes. Possible hormonal regulation was investigated in kidneys using in vivo estradiol treatment, but only slight changes in total apyrase activity were observed.
Subject(s)
Humans , Animals , Rats , Apyrase/metabolism , Kidney/enzymology , Placenta/enzymology , Platelet Aggregation , Apyrase/chemistry , Estradiol/pharmacologyABSTRACT
Se practicaron 96 manometrias anorrectales en ninos con megacolon. En dieciseis los registros eran compatibles con enfermedad de Hirschsprung:en 12 la biopsia rectal por succion para determinar acetilcolinesterasa corroboro el diagnostico de aganglionosis. En 2 pacientes con manometrias no concluyentes, la actividad acetilcolinesterasica resulto altamente positiva,y en uno de ellos se obtuvo el diagnostico de displasia neuronal colonica mediante el estudio de la deshidrogenasa succinica